Regenerative Medicine (Track)
Directing of Neural Differentiation of Cell Transplants
Galina Pavlova
Department of Neural biology
Institute of Gene Biologe, Apto-pharm
Abstract:
Developments of cell therapy could improve treatment of neurodegenerative diseases: after systemic or local introduction of bone marrow cells better regeneration is achieved. It raises hopes for application of autological bone marrow cells and fat cells, which solves both tissue compatibility and ethical problems. Initiated by Korochkin, developments of methods and applications of cells for autological transplantation for animals with experimental neurodegenerative diseases were performed. Methods and factors directing proliferation and differentiation of these cells as culture, and after their transplantation into brain were searched.
The methods are: co-culturing with cells harboring inducing factors, adding of inducing factors into culture medium, transgenic material action on progenitor cell differentiation. Application of conditional mediums for mesenchymal stem cell differentiation toward insulin producing cells was shown. The conditional medium was taken after culturing of corresponding animal organ cells in conventional medium. For directing toward dopaminergic neurons, medium after culturing of substantia nigra of new-born sucking-pig was used.
Another way to improve efficiency of implanted cells is to treat tissue with neurotrophic factors, GDNF or BDNF. Focus is application of stem cells harboring GDNF or BDNF to direct neural differentiation of precursor cells in patient tissues. Res. is supported by Grants RFBR, MSE.
